Dear Access Point,
I am a dialysis nurse who has recently learned therapeutic apheresis. In dialysis, we use membrane technology to separate the waste products from the blood. But in apheresis, we use centrifugation to separate the blood components. I hear people talking about the interface, but I really don’t know what that means. Can you explain?
— Emily in San Francisco, Calif.
Dear Emily,
It’s not uncommon for a new apheresis operator to need more information about all the terms used in apheresis, so here you go: When whole blood separates in the connector of the centrifuge, it separates by specific gravity. Because red blood cells (RBCs) have the highest specific gravity of all the cells, they’re located in the bottom layer. Between the top plasma layer and bottom RBC layer is the buffy coat, containing white blood cells and platelets. The interface is how we describe the line between cells and plasma when blood separates.4
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